Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves.
Identifieur interne : 001760 ( Main/Exploration ); précédent : 001759; suivant : 001761Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves.
Auteurs : Lynn K. Carta [États-Unis] ; Shiguang Li [États-Unis] ; Andrea M. Skantar [États-Unis] ; George Newcombe [États-Unis]Source :
- Journal of nematology [ 0022-300X ] ; 2016.
Abstract
During a long-term, large network study of the ecology of plant endophytes in native habitats, various nematodes have been found. Two poplar species, Populus angustifolia (narrowleaf cottonwood) and Populus trichocarpa (black cottonwood), are important ecological and genomic models now used in ongoing plant-pathogen-endophyte interaction studies. In this study, two different aphelenchid nematodes within surface-sterilized healthy leaves of these two Populus spp. in northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the original Australian type population. The 28S and 18S rRNA trees of Aphelenchoides and Laimaphelenchus species indicated L. heidelbergi failed to cluster with three other Laimaphelenchus species, including the type species of the genus. Therefore, we support a conservative definition of the genus Laimaphelenchus, and consider these populations to belong to Aphelenchoides, amended as Aphelenchoides heidelbergi n. comb. This is the first report of these nematode species from within aboveground leaves. The presence of these fungal-feeding nematodes can affect the balance of endophytic fungi, which are important determinants of plant health.
DOI: 10.21307/jofnem-2017-006
PubMed: 27168650
PubMed Central: PMC4859615
Affiliations:
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Carta</name><affiliation wicri:level="2"><nlm:affiliation>Nematology Laboratory, USDA, ARS, Henry A. Wallace Beltsville Agricultural Research Center, Building 010A, Room 110, Beltsville, MD 20705-2350.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Nematology Laboratory, USDA, ARS, Henry A. Wallace Beltsville Agricultural Research Center, Building 010A, Room 110, Beltsville</wicri:cityArea></affiliation></author><author><name sortKey="Li, Shiguang" sort="Li, Shiguang" uniqKey="Li S" first="Shiguang" last="Li">Shiguang Li</name><affiliation wicri:level="2"><nlm:affiliation>Nematology Laboratory, USDA, ARS, Henry A. Wallace Beltsville Agricultural Research Center, Building 010A, Room 110, Beltsville, MD 20705-2350.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Nematology Laboratory, USDA, ARS, Henry A. 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Wallace Beltsville Agricultural Research Center, Building 010A, Room 110, Beltsville</wicri:cityArea></affiliation></author><author><name sortKey="Newcombe, George" sort="Newcombe, George" uniqKey="Newcombe G" first="George" last="Newcombe">George Newcombe</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Ecology and Biogeosciences, University of Idaho, Moscow, ID 83844.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Idaho</region></placeName><wicri:cityArea>Department of Forest Ecology and Biogeosciences, University of Idaho, Moscow</wicri:cityArea></affiliation></author></analytic><series><title level="j">Journal of nematology</title><idno type="ISSN">0022-300X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">During a long-term, large network study of the ecology of plant endophytes in native habitats, various nematodes have been found. Two poplar species, Populus angustifolia (narrowleaf cottonwood) and Populus trichocarpa (black cottonwood), are important ecological and genomic models now used in ongoing plant-pathogen-endophyte interaction studies. In this study, two different aphelenchid nematodes within surface-sterilized healthy leaves of these two Populus spp. in northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the original Australian type population. The 28S and 18S rRNA trees of Aphelenchoides and Laimaphelenchus species indicated L. heidelbergi failed to cluster with three other Laimaphelenchus species, including the type species of the genus. Therefore, we support a conservative definition of the genus Laimaphelenchus, and consider these populations to belong to Aphelenchoides, amended as Aphelenchoides heidelbergi n. comb. This is the first report of these nematode species from within aboveground leaves. The presence of these fungal-feeding nematodes can affect the balance of endophytic fungi, which are important determinants of plant health. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NASA"><PMID Version="1">27168650</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-300X</ISSN><JournalIssue CitedMedium="Print"><Volume>48</Volume><Issue>1</Issue><PubDate><Year>2016</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of nematology</Title><ISOAbbreviation>J Nematol</ISOAbbreviation></Journal><ArticleTitle>Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves.</ArticleTitle><Pagination><MedlinePgn>28-33</MedlinePgn></Pagination><Abstract><AbstractText>During a long-term, large network study of the ecology of plant endophytes in native habitats, various nematodes have been found. Two poplar species, Populus angustifolia (narrowleaf cottonwood) and Populus trichocarpa (black cottonwood), are important ecological and genomic models now used in ongoing plant-pathogen-endophyte interaction studies. In this study, two different aphelenchid nematodes within surface-sterilized healthy leaves of these two Populus spp. in northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the original Australian type population. The 28S and 18S rRNA trees of Aphelenchoides and Laimaphelenchus species indicated L. heidelbergi failed to cluster with three other Laimaphelenchus species, including the type species of the genus. Therefore, we support a conservative definition of the genus Laimaphelenchus, and consider these populations to belong to Aphelenchoides, amended as Aphelenchoides heidelbergi n. comb. This is the first report of these nematode species from within aboveground leaves. The presence of these fungal-feeding nematodes can affect the balance of endophytic fungi, which are important determinants of plant health. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Carta</LastName><ForeName>Lynn K</ForeName><Initials>LK</Initials><AffiliationInfo><Affiliation>Nematology Laboratory, USDA, ARS, Henry A. 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Carta</name></region><name sortKey="Li, Shiguang" sort="Li, Shiguang" uniqKey="Li S" first="Shiguang" last="Li">Shiguang Li</name><name sortKey="Newcombe, George" sort="Newcombe, George" uniqKey="Newcombe G" first="George" last="Newcombe">George Newcombe</name><name sortKey="Skantar, Andrea M" sort="Skantar, Andrea M" uniqKey="Skantar A" first="Andrea M" last="Skantar">Andrea M. Skantar</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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